PrimePCR™ SYBR® Green Assay: RGD1563812, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RGD1563812, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RGD1563812, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0012261
Assay Design:   exonic
Chromosome Location:   13:100637795-100637953question
Amplicon Length:   129
Splice Variants Targeted:   ENSRNOT00000018982 ENSRNOT00000001975 ENSRNOT00000009893 ENSRNOT00000007957 ENSRNOT00000072768 ENSRNOT00000073718 ENSRNOT00000057655 ENSRNOT00000011646 ENSRNOT00000075216 ENSRNOT00000022864 ENSRNOT00000056735 ENSRNOT00000010989 ENSRNOT00000049582 ENSRNOT00000040306 ENSRNOT00000071383 ENSRNOT00000020785 ENSRNOT00000067416 ENSRNOT00000055597 ENSRNOT00000055114 ENSRNOT00000042547

Gene Information

Description Not Available

Gene Symbol:   RGD1563812
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000031864
Entrez:   498293
Chromosome Mapping:   13q25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.720000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download