PrimePCR™ SYBR® Green Assay: RGD1560718, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RGD1560718, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RGD1560718, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0012186
Assay Design:   exonic
Chromosome Location:   1:56066292-56066441question
Amplicon Length:   120
Splice Variants Targeted:   ENSRNOT00000060924 ENSRNOT00000000574 ENSRNOT00000071131 ENSRNOT00000043852 ENSRNOT00000014202 ENSRNOT00000043162 ENSRNOT00000018234 ENSRNOT00000001826 ENSRNOT00000073144 ENSRNOT00000020722 ENSRNOT00000008508 ENSRNOT00000052098 ENSRNOT00000012162 ENSRNOT00000021134 ENSRNOT00000031015 ENSRNOT00000026631 ENSRNOT00000049492 ENSRNOT00000034426 ENSRNOT00000072895 ENSRNOT00000075019 ENSRNOT00000031973 ENSRNOT00000035813 ENSRNOT00000072055 ENSRNOT00000072276 ENSRNOT00000072662

Gene Information

Description Not Available

Gene Symbol:   RGD1560718
Gene Name:   Sperm motility kinase 2A
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000049155
Entrez:   499027
Chromosome Mapping:   1q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.750000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download