PrimePCR™ SYBR® Green Assay: MARCKSL1, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0011779
Assay Design:   exonic
Chromosome Location:   5:151391201-151391310question
Amplicon Length:   80
Splice Variants Targeted:   ENSRNOT00000066032 ENSRNOT00000002187 ENSRNOT00000051371 ENSRNOT00000040759 ENSRNOT00000048459 ENSRNOT00000049531 ENSRNOT00000043180 ENSRNOT00000045459 ENSRNOT00000017975 ENSRNOT00000047737 ENSRNOT00000023028 ENSRNOT00000024174 ENSRNOT00000075504 ENSRNOT00000042227 ENSRNOT00000016042 ENSRNOT00000030629 ENSRNOT00000043412 ENSRNOT00000052017 ENSRNOT00000041571 ENSRNOT00000009268 ENSRNOT00000051846 ENSRNOT00000012663 ENSRNOT00000016281 ENSRNOT00000073527 ENSRNOT00000071285

Gene Information

Description Not Available

Gene Symbol:   MARCKSL1
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000046423
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.580000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download