PrimePCR™ SYBR® Green Assay: RGD1561815, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0011541
Assay Design:   exonic
Chromosome Location:   2:251713637-251713776question
Amplicon Length:   110
Splice Variants Targeted:   ENSRNOT00000015795 ENSRNOT00000001265 ENSRNOT00000072321 ENSRNOT00000021161 ENSRNOT00000058884 ENSRNOT00000012902 ENSRNOT00000047160 ENSRNOT00000052276 ENSRNOT00000056321 ENSRNOT00000046791 ENSRNOT00000071210 ENSRNOT00000049138 ENSRNOT00000048673 ENSRNOT00000058595 ENSRNOT00000048420 ENSRNOT00000050803 ENSRNOT00000071995 ENSRNOT00000041479 ENSRNOT00000050291 ENSRNOT00000042619 ENSRNOT00000051772 ENSRNOT00000049571 ENSRNOT00000048356 ENSRNOT00000051232 ENSRNOT00000049363 ENSRNOT00000050384 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   RGD1561815
Gene Name:   Protein RGD1561815
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000033461
Entrez:   295472
UniGene:   Not Available
Chromosome Mapping:   2q42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.080000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download