PrimePCR™ SYBR® Green Assay: Dhrsx, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0011066
Assay Design:   exonic
Chromosome Location:   12:20503671-20504232question
Amplicon Length:   61
Splice Variants Targeted:   ENSRNOT00000017730 ENSRNOT00000029485 ENSRNOT00000010025 ENSRNOT00000064428 ENSRNOT00000047701 ENSRNOT00000061742 ENSRNOT00000045320 ENSRNOT00000057062 ENSRNOT00000066004 ENSRNOT00000020316 ENSRNOT00000068360 ENSRNOT00000027182 ENSRNOT00000055137 ENSRNOT00000060412 ENSRNOT00000031770 ENSRNOT00000019309 ENSRNOT00000019304 ENSRNOT00000019652 ENSRNOT00000034159 ENSRNOT00000067217

Gene Information

Description Not Available

Gene Symbol:   Dhrsx
Gene Name:   Dehydrogenase/reductase (SDR family) X chromosome
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000028323
Entrez:   288525
UniGene:   Rn.230398
Chromosome Mapping:   12q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.500000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download