PrimePCR™ SYBR® Green Assay: RGD1564599, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0009717
Assay Design:   exonic
Chromosome Location:   1:149182321-149182467question
Amplicon Length:   117
Splice Variants Targeted:   ENSRNOT00000040301

Gene Information

Description Not Available

Gene Symbol:   RGD1564599
Gene Name:   Uncharacterized protein LOC689600
Aliases:   Not Available
RefSeq:   NM_001173448
Ensembl:   ENSRNOG00000049015
Entrez:   293925
UniGene:   Rn.62289
Chromosome Mapping:   1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.010000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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