PrimePCR™ SYBR® Green Assay: Hist1h2bcl1, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Hist1h2bcl1, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Hist1h2bcl1, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0009683
Assay Design:   exonic
Chromosome Location:   17:58731053-58731281question
Amplicon Length:   199
Splice Variants Targeted:   ENSRNOT00000021405 ENSRNOT00000073712 ENSRNOT00000038175 ENSRNOT00000067060 ENSRNOT00000022432 ENSRNOT00000028779 ENSRNOT00000001387 ENSRNOT00000073931 ENSRNOT00000047596 ENSRNOT00000000662 ENSRNOT00000037877 ENSRNOT00000004406 ENSRNOT00000016423 ENSRNOT00000015963 ENSRNOT00000042070 ENSRNOT00000024581 ENSRNOT00000046129 ENSRNOT00000035512 ENSRNOT00000066190 ENSRNOT00000041187 ENSRNOT00000022630 ENSRNOT00000072195 ENSRNOT00000030198 ENSRNOT00000075811 ENSRNOT00000072293 ENSRNOT00000071688 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   Hist1h2bcl1
Gene Name:   Histone H2B
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000046693
Entrez:   680403
Chromosome Mapping:   17q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.420000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download