PrimePCR™ SYBR® Green Assay: RT1-A2, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0009489
Assay Design:   exonic
Chromosome Location:   20:7433993-7434270question
Amplicon Length:   67
Splice Variants Targeted:   ENSRNOT00000048535 ENSRNOT00000001036 ENSRNOT00000044520 ENSRNOT00000052270 ENSRNOT00000038660

Gene Information

may play a role in antigen presentation [RGD, Feb 2006]

Gene Symbol:   RT1-A2
Gene Name:   RT1 class Ia, locus A2 precursor
Aliases:   Not Available
RefSeq:   NM_001008829
Ensembl:   ENSRNOG00000030712
Entrez:   24974
UniGene:   Rn.116787
Chromosome Mapping:   20p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.150000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download