PrimePCR™ SYBR® Green Assay: Gdf5, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0009390
Assay Design:   exonic
Chromosome Location:   3:157849816-157849916question
Amplicon Length:   71
Splice Variants Targeted:   ENSRNOT00000073736

Gene Information

member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily; regulates cell growth and differentiation; involved with tendon and ligament formation [RGD, Feb 2006]

Gene Symbol:   Gdf5
Gene Name:   Growth and differentiation factor-5
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000050123
Entrez:   252835
UniGene:   Rn.127822
Chromosome Mapping:   3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.590000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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