This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: Pfkfb4, Rat
PrimePCR™ Template for SYBR® Green Assay: Pfkfb4, Rat
has both fructose-2,6-bisphosphate 2-phosphatase and 6-phosphofructo-2-kinase activities; plays a role in fructose 2,6 bisphosphate metabolism and regulation of glycolysis [RGD, Feb 2006]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.