PrimePCR™ SYBR® Green Assay: Cacna2d1, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Cacna2d1, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Cacna2d1, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0006662
Assay Design:   exonic
Chromosome Location:   4:15700171-15700407question
Amplicon Length:   106
Splice Variants Targeted:   ENSRNOT00000073743 ENSRNOT00000072402 ENSRNOT00000034969 ENSRNOT00000042914

Gene Information

subunit of voltage-gated calcium channels; contributes to the regulation of N- and/or L-type calcium channels [RGD, Feb 2006]

Gene Symbol:   Cacna2d1
Gene Name:   Voltage-dependent calcium channel subunit alpha-2/delta-1 isoform 1 precursor
Aliases:   Not Available
RefSeq:   NM_012919 NM_001110847 NM_001110848
Ensembl:   ENSRNOG00000033531
Entrez:   25399
Chromosome Mapping:   4q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.996100
y-intercept 36.840000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download