PrimePCR™ SYBR® Green Assay: Odf2, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Odf2, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Odf2, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0005758
Assay Design:   exonic
Chromosome Location:   3:13809592-13809710question
Amplicon Length:   89
Splice Variants Targeted:   ENSRNOT00000051841 ENSRNOT00000020073 ENSRNOT00000041921 ENSRNOT00000020407 ENSRNOT00000040457 ENSRNOT00000019865

Gene Information

localized to both the cortex and medulla of outer dense fibers, specialized cytoskeletal components of the sperm tail; may interact with other outer dense fiber proteins via a leucine zipper [RGD, Feb 2006]

Gene Symbol:   Odf2
Gene Name:   Outer dense fiber protein 2
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000014584
Entrez:   29479
Chromosome Mapping:   3p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.200000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download