PrimePCR™ SYBR® Green Assay: Prlh, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Prlh, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Prlh, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0005120
Assay Design:   exonic
Chromosome Location:   9:97794510-97795271question
Amplicon Length:   81
Splice Variants Targeted:   ENSRNOT00000007828 ENSRNOT00000068181 ENSRNOT00000011181 ENSRNOT00000010521 ENSRNOT00000061858 ENSRNOT00000026212 ENSRNOT00000030996 ENSRNOT00000064039 ENSRNOT00000068349 ENSRNOT00000042762 ENSRNOT00000047921 ENSRNOT00000022839 ENSRNOT00000026912 ENSRNOT00000073195 ENSRNOT00000015250 ENSRNOT00000026556 ENSRNOT00000061999 ENSRNOT00000027682

Gene Information

Description Not Available

Gene Symbol:   Prlh
Gene Name:   Prolactin-releasing peptide Prolactin-releasing peptide PrRP31 Prolactin-releasing peptide PrRP20
Aliases:   Not Available
RefSeq:   NM_022222
Ensembl:   ENSRNOG00000019871
Entrez:   63850
Chromosome Mapping:   9q36

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 36.130000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download