PrimePCR™ SYBR® Green Assay: Kcnc2, Rat

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0003505
Assay Design:   exonic
Chromosome Location:   7:54999486-54999581question
Amplicon Length:   66
Splice Variants Targeted:   ENSRNOT00000073991 ENSRNOT00000074469 ENSRNOT00000005690 ENSRNOT00000005700 ENSRNOT00000005773 ENSRNOT00000049943

Gene Information

a voltage-gated K+ channel; functions as a delayed rectifier-type K+ channel activated by large membrane depolarizations [RGD, Feb 2006]

Gene Symbol:   Kcnc2
Gene Name:   Potassium voltage-gated channel subfamily C member 2
Aliases:   Not Available
RefSeq:   NM_139216 NM_139217
Ensembl:   ENSRNOG00000004077
Entrez:   246153
UniGene:   Rn.9733
Chromosome Mapping:   7q12-q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.330000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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