PrimePCR™ SYBR® Green Assay: Dync1i2, Rat

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0003358
Assay Design:   exonic
Chromosome Location:   3:64346596-64348680question
Amplicon Length:   120
Splice Variants Targeted:   ENSRNOT00000061884 ENSRNOT00000067729 ENSRNOT00000047243 ENSRNOT00000013400

Gene Information

intermediate chain of cytoplasmic dynein, a retrograde microtubule motor thought to be involved in organelle transport [RGD, Feb 2006]

Gene Symbol:   Dync1i2
Gene Name:   Cytoplasmic dynein 1 intermediate chain 2 isoform 2
Aliases:   Not Available
RefSeq:   NM_053880
Ensembl:   ENSRNOG00000009781
Entrez:   116659
UniGene:   Rn.11014
Chromosome Mapping:   3q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.997600
y-intercept 36.620000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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