PrimePCR™ SYBR® Green Assay: Tpm3, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Tpm3, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Tpm3, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0003186
Assay Design:   exonic
Chromosome Location:   2:208870822-208871008question
Amplicon Length:   64
Splice Variants Targeted:   ENSRNOT00000021073 ENSRNOT00000017339 ENSRNOT00000005073 ENSRNOT00000000655 ENSRNOT00000013286 ENSRNOT00000045270 ENSRNOT00000020669 ENSRNOT00000034672 ENSRNOT00000014249 ENSRNOT00000014388 ENSRNOT00000038160 ENSRNOT00000023567 ENSRNOT00000023605 ENSRNOT00000029076

Gene Information

rod-like protein associated with actin filament in nonmuscle cells; may mediate the stability of actin filaments; may play a role in the regulation of cell transformation, cytokinesis, motility, and morphogenesis [RGD, Feb 2006]

Gene Symbol:   Tpm3
Gene Name:   Tropomyosin alpha-3 chain
Aliases:   Not Available
RefSeq:   NM_173111 NM_057208
Ensembl:   ENSRNOG00000017441
Entrez:   117557
Chromosome Mapping:   2q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.997900
y-intercept 35.550000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download