PrimePCR™ SYBR® Green Assay: Flt3l, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Flt3l, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Flt3l, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0002603
Assay Design:   exonic
Chromosome Location:   1:102195576-102196206question
Amplicon Length:   84
Splice Variants Targeted:   ENSRNOT00000027963 ENSRNOT00000002552 ENSRNOT00000047192 ENSRNOT00000016221 ENSRNOT00000052138 ENSRNOT00000030886 ENSRNOT00000070961 ENSRNOT00000023856 ENSRNOT00000056525 ENSRNOT00000031855 ENSRNOT00000011551 ENSRNOT00000010641 ENSRNOT00000023429 ENSRNOT00000023259 ENSRNOT00000074163 ENSRNOT00000074671 ENSRNOT00000027981 ENSRNOT00000017407 ENSRNOT00000025245 ENSRNOT00000054717 ENSRNOT00000050751 ENSRNOT00000046955

Gene Information

Description Not Available

Gene Symbol:   Flt3l
Gene Name:   FMS-like tyrosine kinase 3 ligand
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000042254
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.540000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download