PrimePCR™ SYBR® Green Assay: Rpl27, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0001743
Assay Design:   exonic
Chromosome Location:   10:89151899-89154291question
Amplicon Length:   109
Splice Variants Targeted:   ENSRNOT00000057689 ENSRNOT00000071321 ENSRNOT00000028060 ENSRNOT00000047308 ENSRNOT00000042756 ENSRNOT00000073647 ENSRNOT00000071909 ENSRNOT00000057891

Gene Information

ribosomal protein [RGD, Feb 2006]

Gene Symbol:   Rpl27
Gene Name:   60S ribosomal protein L27
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000020674
Entrez:   64306
UniGene:   Rn.1254
Chromosome Mapping:   10q32.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.390000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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