PrimePCR™ Probe Assay: Zfp931, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Zfp931, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Zfp931, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0043646
Assay Design:   Intron-spanning
Chromosome Location:   2:178069871-178078411question
Amplicon Length:   103
Splice Variants Targeted:   ENSMUST00000084501 ENSMUST00000109062 ENSMUST00000109060 ENSMUST00000109059 ENSMUST00000099028 ENSMUST00000109056 ENSMUST00000109058 ENSMUST00000109055 ENSMUST00000109051 ENSMUST00000121393 ENSMUST00000131676 ENSMUST00000109047 ENSMUST00000109045 ENSMUST00000152941 ENSMUST00000109042 ENSMUST00000109002 ENSMUST00000143490 ENSMUST00000179061 ENSMUST00000131041 ENSMUST00000109020 ENSMUST00000109011 ENSMUST00000108949 ENSMUST00000109007 ENSMUST00000174416 ENSMUST00000099009 ENSMUST00000136719 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Zfp931
Gene Name:   Zinc finger protein 931
Aliases:   2810021G02Rik
RefSeq:   NC_000068.7 NT_039212.7
Ensembl:   ENSMUSG00000078861
Entrez:   353208
Chromosome Mapping:   2 H4|2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 38.880000
Efficiency 88

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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