PrimePCR™ Probe Assay: Tom1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Tom1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Tom1, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0043153
Assay Design:   Intron-spanning
Chromosome Location:   8:75067524-75069128question
Amplicon Length:   61
Splice Variants Targeted:   ENSMUST00000061620 ENSMUST00000058440 ENSMUST00000052138 ENSMUST00000078847 ENSMUST00000165630 ENSMUST00000027965 ENSMUST00000126977 ENSMUST00000143254 ENSMUST00000140448 ENSMUST00000127297 ENSMUST00000025079 ENSMUST00000034375 ENSMUST00000119736 ENSMUST00000053215 ENSMUST00000085469 ENSMUST00000036712 ENSMUST00000042471 ENSMUST00000159584 ENSMUST00000088827 ENSMUST00000043911 ENSMUST00000000985 ENSMUST00000170489 ENSMUST00000078961 ENSMUST00000006614 ENSMUST00000108836 ENSMUST00000058704 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Tom1
Gene Name:   Target of myb1 homolog (chicken)
Aliases:   Not Available
RefSeq:   NC_000074.6 NT_078575.7
Ensembl:   ENSMUSG00000042870
Entrez:   21968
Chromosome Mapping:   8 C1|8

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.660000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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