PrimePCR™ Probe Assay: Tfrc, Mouse

print
RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:  
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0041876
Assay Design:   intron_spanning
Chromosome Location:   16:32613193-32614665question
Amplicon Length:   104
Splice Variants Targeted:   ENSMUST00000023486 ENSMUST00000120680

Gene Information

This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Mice that are deficient in this receptor show impaired erythroid development and abnormal iron homeostasis. [provided by RefSeq Mar 2010]

Gene Symbol:   Tfrc
Gene Name:   Transferrin receptor
Aliases:   2610028K12Rik, AI195355, AI426448, AU015758, CD71, E430033M20Rik, Mtvr-1, Mtvr1, TFR, TFR1, TR, Trfr, p90
RefSeq:   NC_000082.6 NT_039624.8
Ensembl:   ENSMUSG00000022797
Entrez:   22042
UniGene:   Mm.28683
Chromosome Mapping   16 B3|16 21.2 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.070000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev C Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev D Click to download
Get Help

Call us at 1-800-4-BIORAD (1-800-424-6723)

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.