PrimePCR™ Probe Assay: Wapal, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0035640
Assay Design:   Intron-spanning
Chromosome Location:   14:34739379-34741129question
Amplicon Length:   103
Splice Variants Targeted:   ENSMUST00000048263 ENSMUST00000090027 ENSMUST00000151285 ENSMUST00000174559 ENSMUST00000169910

Gene Information

Description Not Available

Gene Symbol:   Wapal
Gene Name:   Wings apart-like homolog (Drosophila)
Aliases:   2410073G16, A530089A20Rik, BC037674, FOE, mKIAA0261, mWAPL
RefSeq:   NC_000080.6 NT_039606.8
Ensembl:   ENSMUSG00000041408
Entrez:   218914
UniGene:   Mm.27957
Chromosome Mapping:   14 B|14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 34.570000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download