PrimePCR™ Probe Assay: Park2, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0034824
Assay Design:   Intron-spanning
Chromosome Location:   17:10840392-10940153question
Amplicon Length:   147
Splice Variants Targeted:   ENSMUST00000097413 ENSMUST00000174252 ENSMUST00000092227

Gene Information

Description Not Available

Gene Symbol:   Park2
Gene Name:   Parkinson disease (autosomal recessive, juvenile) 2, parkin
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000073465
UniGene:   Mm.311110
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 44.620000
Efficiency 74

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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