PrimePCR™ Probe Assay: 2510002D24Rik, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0034492
Assay Design:   Intron-spanning
Chromosome Location:   16:18836740-18839156question
Amplicon Length:   171
Splice Variants Targeted:   ENSMUST00000055413

Gene Information

Description Not Available

Gene Symbol:   2510002D24Rik
Gene Name:   RIKEN cDNA 2510002D24 gene
Aliases:   2410043I01Rik, AA163535, AA409624, DXMgi27
RefSeq:   NC_000082.6 NT_039624.8 NT_187008.1
Ensembl:   ENSMUSG00000071632
Entrez:   72307
UniGene:   Mm.36907
Chromosome Mapping:   16 A3|16

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.100000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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