PrimePCR™ Probe Assay: Zfp426, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0034314
Assay Design:   Intron-spanning
Chromosome Location:   9:20474940-20476440question
Amplicon Length:   109
Splice Variants Targeted:   ENSMUST00000115562 ENSMUST00000169558 ENSMUST00000080386 ENSMUST00000163348 ENSMUST00000164825 ENSMUST00000164799 ENSMUST00000169269 ENSMUST00000166005

Gene Information

Description Not Available

Gene Symbol:   Zfp426
Gene Name:   Zinc finger protein 426
Aliases:   2900057C04Rik, KRAB1, Zfo61, Zfp68-rs1, Znf426, mKIAA4237
RefSeq:   NC_000075.6 NT_039472.8
Ensembl:   ENSMUSG00000059475
Entrez:   235028
UniGene:   Mm.297660
Chromosome Mapping:   9 A3|9 37.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.020000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download