This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: Pik3r6, Mouse
PrimePCR™ Template for Probe Assay: Pik3r6, Mouse
Phosphoinositide 3-kinase gamma is a lipid kinase that produces the lipid second messenger phosphatidylinositol 345-trisphosphate. The kinase is composed of a catalytic subunit and one of several regulatory subunits and is chiefly activated by G protein-coupled receptors. This gene encodes a regulatory subunit and is distantly related to the phosphoinositide-3-kinase regulatory subunit 5 gene which is located adjacent to this gene on chromosome 11. The protein binds to both the catalytic subunit and to G(beta/gamma) and mediates activation of the kinase subunit downstream of G protein-coupled receptors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.