PrimePCR™ Probe Assay: Pik3r6, Mouse

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0033872
Assay Design:   Intron-spanning
Chromosome Location:   11:68545888-68551296question
Amplicon Length:   71
Splice Variants Targeted:   ENSMUST00000102613 ENSMUST00000060441

Gene Information

Phosphoinositide 3-kinase gamma is a lipid kinase that produces the lipid second messenger phosphatidylinositol 345-trisphosphate. The kinase is composed of a catalytic subunit and one of several regulatory subunits and is chiefly activated by G protein-coupled receptors. This gene encodes a regulatory subunit and is distantly related to the phosphoinositide-3-kinase regulatory subunit 5 gene which is located adjacent to this gene on chromosome 11. The protein binds to both the catalytic subunit and to G(beta/gamma) and mediates activation of the kinase subunit downstream of G protein-coupled receptors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   Pik3r6
Gene Name:   Phosphoinositide-3-kinase, regulatory subunit 6
Aliases:   BB220380, p84, p87(PIKAP), p87PIKAP
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000046207
Entrez:   104709
UniGene:   Mm.234573
Chromosome Mapping:   11 B3|11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.540000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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