PrimePCR™ Probe Assay: Fam102a, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0033088
Assay Design:   Intron-spanning
Chromosome Location:   2:32536113-32558078question
Amplicon Length:   142
Splice Variants Targeted:   ENSMUST00000048375 ENSMUST00000133366

Gene Information

Description Not Available

Gene Symbol:   Fam102a
Gene Name:   Family with sequence similarity 102, member A
Aliases:   AI426465, C230093N12Rik, Eeig1
RefSeq:   NC_000068.7 NT_039206.8
Ensembl:   ENSMUSG00000039157
Entrez:   98952
UniGene:   Mm.4065
Chromosome Mapping:   2 B|2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 34.830000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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