PrimePCR™ Probe Assay: Eci2, Mouse

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0032712
Assay Design:   Intron-spanning
Chromosome Location:   13:34990755-34993151question
Amplicon Length:   116
Splice Variants Targeted:   ENSMUST00000171229 ENSMUST00000021854 ENSMUST00000110251 ENSMUST00000170538 ENSMUST00000163280 ENSMUST00000170989 ENSMUST00000167036 ENSMUST00000171258 ENSMUST00000169759 ENSMUST00000178421

Gene Information

Description Not Available

Gene Symbol:   Eci2
Gene Name:   Enoyl-Coenzyme A delta isomerase 2
Aliases:   Acbd2, Drs1, Hca88, Peci
RefSeq:   NC_000079.6 NT_039578.8
Ensembl:   ENSMUSG00000021417
Entrez:   23986
UniGene:   Mm.28883
Chromosome Mapping:   13|13 A4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.130000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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