This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: Thtpa, Mouse
PrimePCR™ Template for Probe Assay: Thtpa, Mouse
This gene encodes a cytosolic enzyme that catalyzes the conversion of thiamine triphosphate to thiamine diphosphate. The encoded protein requires divalent cations for enzymatic activity. The gene product is believed to play a role in regulation of thiamine triphosphate intracellular concentration and it may be a messenger in cell signaling in response to cellular stress. [provided by RefSeq Mar 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.