PrimePCR™ Probe Assay: 4930504O13Rik, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0032561
Assay Design:   Intron-spanning
Chromosome Location:   11:58446324-58447379question
Amplicon Length:   107
Splice Variants Targeted:   ENSMUST00000064614

Gene Information

Description Not Available

Gene Symbol:   4930504O13Rik
Gene Name:   RIKEN cDNA 4930504O13 gene
Aliases:   Gm524
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000052642
Entrez:   403200
UniGene:   Mm.60809
Chromosome Mapping:   11 B1.3|11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.260000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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