PrimePCR™ Probe Assay: Anapc2, Mouse

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0032528
Assay Design:   Intron-spanning
Chromosome Location:   2:25274774-25276397question
Amplicon Length:   119
Splice Variants Targeted:   ENSMUST00000028341 ENSMUST00000129300

Gene Information

This gene encodes a component of the anaphase promoting complex/cyclosome (APC/C) a cell cycle-regulated ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor for subsequent degradation by the proteasome. [provided by RefSeq Oct 2009]

Gene Symbol:   Anapc2
Gene Name:   Anaphase promoting complex subunit 2
Aliases:   9230107K09Rik, AL024279, Apc2, Emi4, Imi4, mKIAA1406
RefSeq:   NC_000068.7 NT_039206.8
Ensembl:   ENSMUSG00000026965
Entrez:   99152
UniGene:   Mm.291624
Chromosome Mapping:   2 A3|2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.880000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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