PrimePCR™ Probe Assay: Trp53, Mouse

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0032520
Assay Design:   Intron-spanning
Chromosome Location:   11:69580402-69587378question
Amplicon Length:   195
Splice Variants Targeted:   ENSMUST00000005371 ENSMUST00000108657 ENSMUST00000108658 ENSMUST00000171247

Gene Information

This gene encodes tumor protein p53 which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest apoptosis senescence DNA repair or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation DNA-binding and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion and thus function as a tumor suppressor. Mice deficient for this gene are developmentally normal but are susceptible to spontaneous tumors. Evidence to date shows that this gene contains one promoter in contrast to alternative promoters of the human gene and transcribes a few of splice variants which encode different isoforms although the biological validity or the full-length nature of some variants has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   Trp53
Gene Name:   Transformation related protein 53
Aliases:   Tp53, bbl, bfy, bhy, p44, p53
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000059552
Entrez:   22059
UniGene:   Mm.222
Chromosome Mapping:   11 B2-C|11 39.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.110000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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