PrimePCR™ Probe Assay: Mid2, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0032355
Assay Design:   Intron-spanning
Chromosome Location:   X:140678598-140693096question
Amplicon Length:   114
Splice Variants Targeted:   ENSMUST00000112990 ENSMUST00000112988 ENSMUST00000112993

Gene Information

Description Not Available

Gene Symbol:   Mid2
Gene Name:   Midline 2
Aliases:   FXY2, Trim1
RefSeq:   NC_000086.7 NT_039716.8
Ensembl:   ENSMUSG00000000266
Entrez:   23947
UniGene:   Mm.131097
Chromosome Mapping:   X F1|X 60.5 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 34.960000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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