PrimePCR™ Probe Assay: Zc3h12b, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0032267
Assay Design:   Intron-spanning
Chromosome Location:   X:95925315-95926607question
Amplicon Length:   84
Splice Variants Targeted:   ENSMUST00000127461 ENSMUST00000082183

Gene Information

Description Not Available

Gene Symbol:   Zc3h12b
Gene Name:   Zinc finger CCCH-type containing 12B
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000035045
UniGene:   Mm.302708
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 33.730000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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