PrimePCR™ Probe Assay: Pml, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0031710
Assay Design:   Intron-spanning
Chromosome Location:   9:58221839-58229888question
Amplicon Length:   111
Splice Variants Targeted:   ENSMUST00000085673 ENSMUST00000114136 ENSMUST00000135310 ENSMUST00000126690 ENSMUST00000153820

Gene Information

Description Not Available

Gene Symbol:   Pml
Gene Name:   Promyelocytic leukemia
Aliases:   1200009E24Rik, AI661194, Trim19
RefSeq:   NC_000075.6 NT_039474.8
Ensembl:   ENSMUSG00000036986
Entrez:   18854
Chromosome Mapping:   9 B|9 32.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.700000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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