This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: Add2, Mouse
PrimePCR™ Template for Probe Assay: Add2, Mouse
This gene encodes the beta subunit of the adducin family. Adducins encoded by alpha beta and gamma genes are heteromeric proteins that crosslink actin filaments with spectrin at the cytoskeletal membrane. This protein primarily found in the brain and hematopoietic cells is regulated by phosphorylation and calmodulin interactions as it promotes spectrin assembly onto actin filaments bundles actin and caps barbed ends of actin filaments. In mouse deficiency of this gene can lead to mild hemolytic anemia and impaired synaptic plasticity. Mutations of this gene in mouse serve as a pathophysiological model for hereditary spherocytosis and hereditary elliptocytosis. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq Dec 2012]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.