PrimePCR™ Probe Assay: Add2, Mouse


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0031537
Assay Design:   Intron-spanning
Chromosome Location:   6:86109796-86110855question
Amplicon Length:   73
Splice Variants Targeted:   ENSMUST00000032069

Gene Information

This gene encodes the beta subunit of the adducin family. Adducins encoded by alpha beta and gamma genes are heteromeric proteins that crosslink actin filaments with spectrin at the cytoskeletal membrane. This protein primarily found in the brain and hematopoietic cells is regulated by phosphorylation and calmodulin interactions as it promotes spectrin assembly onto actin filaments bundles actin and caps barbed ends of actin filaments. In mouse deficiency of this gene can lead to mild hemolytic anemia and impaired synaptic plasticity. Mutations of this gene in mouse serve as a pathophysiological model for hereditary spherocytosis and hereditary elliptocytosis. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq Dec 2012]

Gene Symbol:   Add2
Gene Name:   Adducin 2 (beta)
Aliases:   2900072M03Rik
RefSeq:   NC_000072.6 NT_039353.8
Ensembl:   ENSMUSG00000030000
Entrez:   11519
UniGene:   Mm.104155
Chromosome Mapping:   6 D1|6 35.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.520000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download