PrimePCR™ Probe Assay: Irak1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0030079
Assay Design:   Intron-spanning
Chromosome Location:   X:74018312-74020354question
Amplicon Length:   118
Splice Variants Targeted:   ENSMUST00000114354 ENSMUST00000114352 ENSMUST00000068286 ENSMUST00000114360 ENSMUST00000033769 ENSMUST00000101458 ENSMUST00000126583

Gene Information

Description Not Available

Gene Symbol:   Irak1
Gene Name:   Interleukin-1 receptor-associated kinase 1
Aliases:   AA408924, IRAK, IRAK-1, IRAK1-S, Il1rak, Plpk, mPLK
RefSeq:   NC_000086.7 NT_039706.8
Ensembl:   ENSMUSG00000031392
Entrez:   16179
UniGene:   Mm.38241
Chromosome Mapping:   X A7.3|X 29.6 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.670000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download