PrimePCR™ Probe Assay: Aspa, Mouse

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0030028
Assay Design:   Intron-spanning
Chromosome Location:   11:73319857-73322193question
Amplicon Length:   110
Splice Variants Targeted:   ENSMUST00000021119 ENSMUST00000134079

Gene Information

This gene encodes an enzyme that deacteylates N-acetyl-L-aspartic acid (NAA) in the brain to yield acetate and L-aspartate. In humans alterations in neuronal NAA concentration are associated with many neurodegenerative diseases (decrease associated with epilepsy multiple sclerosis myotrophic lateral sclerosis and Alzheimer's disease; increase associated with Canavan disease). In mouse mutations in this gene which cause accumulation of NAA result in demyelination and spongy degeneration in the CNS and serve as a pathophysiological model for Canavan disease. [provided by RefSeq Dec 2012]

Gene Symbol:   Aspa
Gene Name:   Aspartoacylase
Aliases:   Acy-2, Acy2, nur7
RefSeq:   NC_000077.6 NT_096135.6 NT_166317.2
Ensembl:   ENSMUSG00000020774
Entrez:   11484
UniGene:   Mm.293574
Chromosome Mapping:   11 B4|11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.180000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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