PrimePCR™ Probe Assay: Dbndd1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0029499
Assay Design:   Intron-spanning
Chromosome Location:   8:123509937-123515344question
Amplicon Length:   140
Splice Variants Targeted:   ENSMUST00000176286 ENSMUST00000177240 ENSMUST00000176155 ENSMUST00000169210 ENSMUST00000074879 ENSMUST00000066198

Gene Information

Description Not Available

Gene Symbol:   Dbndd1
Gene Name:   Dysbindin (dystrobrevin binding protein 1) domain containing 1
Aliases:   2810427I04Rik, D8Ertd590e
RefSeq:   NC_000074.6 NT_078575.7
Ensembl:   ENSMUSG00000031970
Entrez:   72185
UniGene:   Mm.27925
Chromosome Mapping:   8 E2|8 67.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.960000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download