PrimePCR™ Probe Assay: Nppc, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Nppc, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Nppc, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0029238
Assay Design:   Intron-spanning
Chromosome Location:   1:86666648-86669700question
Amplicon Length:   178
Splice Variants Targeted:   ENSMUST00000027449

Gene Information

This gene encodes a member of the natriuretic peptide family. Natriuretic peptides are involved in the control of blood pressure extracellular fluid volume and electrolyte homeostasis. The encoded protein also plays a role in sensory neuron bifurcation and is a critical regulator of endochondral bone growth. The encoded protein is a ligand for the natriuretic peptide receptor B and is synthesized as a preprohormone which is cleaved to produce a mature peptide. Mutations in this gene are associated with dwarfism resulting from impaired endochondral ossification. [provided by RefSeq Apr 2011]

Gene Symbol:   Nppc
Gene Name:   Natriuretic peptide type C
Aliases:   CNP, lbab
RefSeq:   NC_000067.6 NT_078297.7
Ensembl:   ENSMUSG00000026241
Entrez:   18159
Chromosome Mapping:   1 D|1 54.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.330000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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