PrimePCR™ Probe Assay: Mtap2, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0028445
Assay Design:   Intron-spanning
Chromosome Location:   1:66420155-66421788question
Amplicon Length:   155
Splice Variants Targeted:   ENSMUST00000114017 ENSMUST00000114015 ENSMUST00000114013 ENSMUST00000077355 ENSMUST00000114012 ENSMUST00000172886 ENSMUST00000173778 ENSMUST00000173800 ENSMUST00000114018 ENSMUST00000024639

Gene Information

Description Not Available

Gene Symbol:   Mtap2
Gene Name:   Microtubule-associated protein 2
Aliases:   MAP-2, MAP2, Mtap-2
RefSeq:   NC_000067.6 NT_039170.8 NT_187020.1
Ensembl:   ENSMUSG00000015222
Entrez:   17756
UniGene:   Mm.256966
Chromosome Mapping:   1 C3|1 33.7 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 34.950000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download