PrimePCR™ Probe Assay: Shc1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0028356
Assay Design:   Intron-spanning
Chromosome Location:   3:89424794-89426564question
Amplicon Length:   119
Splice Variants Targeted:   ENSMUST00000039110 ENSMUST00000125036 ENSMUST00000128238 ENSMUST00000107417 ENSMUST00000094378 ENSMUST00000137793

Gene Information

Description Not Available

Gene Symbol:   Shc1
Gene Name:   Src homology 2 domain-containing transforming protein C1
Aliases:   Shc, ShcA, p66, p66shc
RefSeq:   NC_000069.6 NT_039240.8
Ensembl:   ENSMUSG00000042626
Entrez:   20416
UniGene:   Mm.86595
Chromosome Mapping:   3 F1|3 45.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.910000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download