PrimePCR™ Probe Assay: Fryl, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0028309
Assay Design:   Intron-spanning
Chromosome Location:   5:73119840-73121933question
Amplicon Length:   117
Splice Variants Targeted:   ENSMUST00000101127 ENSMUST00000152631 ENSMUST00000094700

Gene Information

Description Not Available

Gene Symbol:   Fryl
Gene Name:   Furry homolog-like (Drosophila)
Aliases:   2010313D22Rik, 2310004H21Rik, 2510002A14Rik, 9030227G01Rik, mKIAA0826
RefSeq:   NC_000071.6 NT_039305.8
Ensembl:   ENSMUSG00000070733
Entrez:   72313
UniGene:   Mm.490279
Chromosome Mapping:   5 C3.2|5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.430000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download