PrimePCR™ SYBR® Green Assay: Sult2a2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qMmuCIP0062893

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0061744
Assay Design:   Intron-spanning
Chromosome Location:   7:13738303-13779515question
Amplicon Length:   113
Splice Variants Targeted:   ENSMUST00000108525 ENSMUST00000086148 ENSMUST00000108522 ENSMUST00000108520 ENSMUST00000165167 ENSMUST00000098809 ENSMUST00000108524 ENSMUST00000124729

Gene Information

This is one of seven sulfotransferase family 2A genes in a chromosome 7 A1 cluster. [provided by RefSeq May 2010]

Gene Symbol:   Sult2a2
Gene Name:   Sulfotransferase family 2A, dehydroepiandrosterone (DHEA)-preferring, member 2
Aliases:   C730007P19Rik, ST, ST2A2, Sth2, mSTa2
RefSeq:   NC_000073.6 NT_187034.1
Ensembl:   ENSMUSG00000070811
Entrez:   100043194
UniGene:   Mm.457986
Chromosome Mapping:   7 A1|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.000000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download