PrimePCR™ SYBR® Green Assay: Cyp2c40, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qMmuCIP0062871

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0061722
Assay Design:   Intron-spanning
Chromosome Location:   19:39786838-39802640question
Amplicon Length:   194
Splice Variants Targeted:   ENSMUST00000067328 ENSMUST00000099472 ENSMUST00000160476 ENSMUST00000168838 ENSMUST00000049178 ENSMUST00000048959 ENSMUST00000080171

Gene Information

Description Not Available

Gene Symbol:   Cyp2c40
Gene Name:   Cytochrome P450, family 2, subfamily c, polypeptide 40
Aliases:   AI265721, AI662255
RefSeq:   NC_000085.6 NT_039687.8
Ensembl:   ENSMUSG00000025004
Entrez:   13099
UniGene:   Mm.353875
Chromosome Mapping:   19 C3|19

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.320000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download