PrimePCR™ SYBR® Green Assay: Zfp534, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0062858

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0061709
Assay Design:   Intron-spanning
Chromosome Location:   4:147678332-147702524question
Amplicon Length:   159
Splice Variants Targeted:   ENSMUST00000105742 ENSMUST00000136309 ENSMUST00000105741 ENSMUST00000105738 ENSMUST00000123460 ENSMUST00000105730 ENSMUST00000091878 ENSMUST00000168483 ENSMUST00000105735 ENSMUST00000140089 ENSMUST00000179175 ENSMUST00000049821 ENSMUST00000146688 ENSMUST00000075775 ENSMUST00000148762 ENSMUST00000133078 ENSMUST00000154154 ENSMUST00000105721 ENSMUST00000139784 ENSMUST00000143885 ENSMUST00000081742 ENSMUST00000105720 ENSMUST00000133006 ENSMUST00000037565 ENSMUST00000117638 ENSMUST00000122309

Gene Information

Description Not Available

Gene Symbol:   Zfp534
Gene Name:   Zinc finger protein 534
Aliases:   Gm13159, OTTMUSG00000010681, Rp23-282c23.3
RefSeq:   NT_166299.2 NC_000070.6 NT_187033.1
Ensembl:   ENSMUSG00000062518
Entrez:   100503584
UniGene:   Not Available
Chromosome Mapping:   4 E2|4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.970000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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