PrimePCR™ SYBR® Green Assay: Vmn2r67, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qMmuCIP0062827

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0061678
Assay Design:   Intron-spanning
Chromosome Location:   7:85137112-85149952question
Amplicon Length:   109
Splice Variants Targeted:   ENSMUST00000124773 ENSMUST00000168730 ENSMUST00000061074 ENSMUST00000171213 ENSMUST00000168230 ENSMUST00000172338 ENSMUST00000063425 ENSMUST00000077478 ENSMUST00000166355 ENSMUST00000167830

Gene Information

Description Not Available

Gene Symbol:   Vmn2r67
Gene Name:   Vomeronasal 2, receptor 67
Aliases:   EG620672
RefSeq:   NC_000073.6 NT_039433.8
Ensembl:   ENSMUSG00000095664
Entrez:   620672
UniGene:   Mm.469836
Chromosome Mapping:   7 D3|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.470000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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