PrimePCR™ SYBR® Green Assay: Vmn2r47, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qMmuCIP0062795

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Vmn2r47, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Vmn2r47, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0061646
Assay Design:   Intron-spanning
Chromosome Location:   7:9829801-9838020question
Amplicon Length:   120
Splice Variants Targeted:   ENSMUST00000086297 ENSMUST00000170922 ENSMUST00000174368 ENSMUST00000072475 ENSMUST00000075108 ENSMUST00000094866 ENSMUST00000165921 ENSMUST00000173459 ENSMUST00000169683 ENSMUST00000170402 ENSMUST00000168807 ENSMUST00000086282 ENSMUST00000146278 ENSMUST00000142934 ENSMUST00000166499 ENSMUST00000164845 ENSMUST00000171942 ENSMUST00000169206 ENSMUST00000174388 ENSMUST00000108554 ENSMUST00000072787 ENSMUST00000174433 ENSMUST00000171523 ENSMUST00000170131 ENSMUST00000165611 ENSMUST00000108550 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Vmn2r47
Gene Name:   Vomeronasal 2, receptor 47
Aliases:   Not Available
RefSeq:   NC_000073.6 NT_187034.1
Ensembl:   ENSMUSG00000094107
Entrez:   100042891
Chromosome Mapping:   7 A1|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.460000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download