PrimePCR™ SYBR® Green Assay: Fbl, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0062760

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Fbl, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Fbl, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0061611
Assay Design:   Intron-spanning
Chromosome Location:   7:28169774-28174608question
Amplicon Length:   120
Splice Variants Targeted:   ENSMUST00000092723 ENSMUST00000115797 ENSMUST00000116547 ENSMUST00000098789 ENSMUST00000042405 ENSMUST00000057801 ENSMUST00000117282 ENSMUST00000085383 ENSMUST00000037012 ENSMUST00000105600 ENSMUST00000154166 ENSMUST00000024756 ENSMUST00000108638 ENSMUST00000159861 ENSMUST00000084299

Gene Information

Description Not Available

Gene Symbol:   Fbl
Gene Name:   Fibrillarin
Aliases:   AL022665, FIB, FLRN, RNU3IP1
RefSeq:   NC_000073.6 NT_187034.1
Ensembl:   ENSMUSG00000046865
Entrez:   14113
Chromosome Mapping:   7 A1|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.600000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download