PrimePCR™ SYBR® Green Assay: Mup1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qMmuCIP0062726

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0061577
Assay Design:   Intron-spanning
Chromosome Location:   4:60500472-60501367question
Amplicon Length:   97
Splice Variants Targeted:   ENSMUST00000075973 ENSMUST00000107521 ENSMUST00000107517 ENSMUST00000107520 ENSMUST00000079697 ENSMUST00000125282 ENSMUST00000166098 ENSMUST00000074700 ENSMUST00000107490 ENSMUST00000095058 ENSMUST00000163931 ENSMUST00000122381 ENSMUST00000118759 ENSMUST00000107506 ENSMUST00000122177 ENSMUST00000103012 ENSMUST00000084548 ENSMUST00000107499 ENSMUST00000098047 ENSMUST00000098046 ENSMUST00000117932 ENSMUST00000072678 ENSMUST00000098042 ENSMUST00000125461 ENSMUST00000075206 ENSMUST00000071005 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Mup1
Gene Name:   Major urinary protein 1
Aliases:   Ltn-1, Lvtn-1, Mup-1, Mup-a, Mup10, Mup7, Up-1
RefSeq:   NC_000070.6 NT_187032.1
Ensembl:   ENSMUSG00000078683
Entrez:   17840
UniGene:   Mm.489589
Chromosome Mapping:   4 B3|4 27.8 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.170000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download